鄂尔多斯站博士生王佳肉苁蓉种子休眠和萌发特性论文被Plant Physiology & Biochemistry接受发表

作者：鄂尔多斯生态站   更新时间：2017-04-17

 Wang, J., Baskin, J. M., Baskin, C. C., Liu, G. F., Yang, X. J., Huang, Z. Y., 2017. Seed dormancy and germination of the medicinal holoparasitic plant Cistanche deserticola from the cold desert of northwest china. Plant Physiology & Biochemistry, 115, 279-285.

Title：Seed dormancy and germination of the medicinal holoparasitic plant Cistanche deserticola from the cold desert of northwest China

题目：中国西北部冷沙漠药用植物肉苁蓉种子的休眠和萌发特性

Abstract:

Cistanche deserticola is a holoparasitic plant with high medicinal value that reproduces only by seeds. However, the requirements for seed dormancy break and germination of this species remain unclear. The freshly matured dust-like seeds consist of a water-permeable seed coat and an undifferentiated oval-shaped embryo embedded in endosperm. No fresh seeds germinated in water or a 10^-5 Mfluridone solution at any incubation temperature within 60 days. Length of embryos in seeds incubated in warm- and cold-started stratification sequences had increased 10.4 and 11.7 % after 50 and 40 weeks, respectively. After 6 months, length of embryos in seeds stratified at 5 ºC had increased by 12 %. Germination of fresh seeds and of seeds stratified at 5 ºC for 6 months and then incubated in mixed fluridone/gibberellic acid 3 (GA₃) solutions at 30/20 ºC germinated to only 2.6 and 11.7 %, respectively. Embryos of fresh seeds and of cold-stratified seeds had increased 29.4 and 15.8 % in length, respectively, at the time of germination, but they never differentiated into organs. The highest germination (54.4 %) was for seeds incubated in a 10^{-5 M}solution of fluridone in darkness in spring that had overwinter on the soil surface in the natural habitat. Our study indicates that breaking of physiological dormancy (PD) occurs first and then the embryo grows to a critical length (0.44 mm) without differentiation into organs prior to seed germination. Seeds for which PD had been broken were induced to germinate by fluridone and GA₃ at high temperature. Taken together, these results suggest that C. deserticola seeds have a specialized kind of morphophysiological dormancy. This study reveals possible ways to release seed dormancy that will be useful in propagating this medicinal species.

中文摘要：

肉苁蓉是一类仅靠种子进行繁殖、且具有高药用价值的全寄生植物。然而，该植物种子休眠释放和萌发的条件仍不清楚。新鲜成熟的灰尘状种子由透水的种皮和包裹在胚乳中的椭圆型胚组成。新成熟种子在任何温度下的蒸馏水或10^-5M氟啶酮溶液中培养60天无萌发现象出现。经温和冷起始的层积组合处理50和40周后，胚长度分别增加了10.4%和11.7%。经5℃层积6个月后，种胚长度增加了12%。新成熟种子和经5℃层积6个月后的种子，在30/20℃、氟啶酮/赤霉素混合溶液中培养6个月后的萌发率分别为2.6%和11.7%。在萌发起始时，新成熟种子和经冷层积处理种子的胚长度分别增加了29.4%和15.8%；然而，种胚未分化成任何器官。自然生境沙土表面的种子，在经历冬季后，于春季获得最高的种子萌发率（54.4%）。我们的研究表明种子萌发需要两个条件：首先，种子释放生理休眠（PD）；其次，胚生长至一个关键长度（0.44mm），胚未分化成任何器官。已释放PD的种子能在高温下被氟啶酮和赤霉素混合溶液诱导萌发。总之，这些研究表明肉苁蓉种子具有特化的形态生理休眠（MPD）。本研究揭示了肉苁蓉种子休眠释放的可能途径，这将有利于该药用植物的繁殖。